High-throughput and ultra-sensitive single-cell profiling of multiple microRNAs and identification of human cancer

Lu Li; Meng Lu; Yuanyuan Fan; Lingling Shui; Shuting Xie; Renjie Sheng; Haibin Si; Qingling Li; Yiguo Wang; Bo Tang

doi:10.1039/C9CC05553C

High-throughput and ultra-sensitive single-cell profiling of multiple microRNAs and identification of human cancer†

Lu Li,^a Meng Lu,^a Yuanyuan Fan,^a Lingling Shui,

^b Shuting Xie,^b Renjie Sheng,^a Haibin Si,^a Qingling Li,^a Yiguo Wang^c and Bo Tang

*^a

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* Corresponding authors

^a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education Shandong Normal University, Jinan 250014, Shandong, P. R. China
E-mail: tangb@sdnu.edu.cn

^b Guangdong Provincial Key Laboratory of Optical Information Materials and Technology and Institute of Electronic Paper Displays, South China Academy of Advanced Optoelectronics, South China Normal University, Guangzhou 510006, Guangdong, P. R. China

^c Shandong Provincial Qianfoshan Hospital, the Frist Hospital Affiliated with Shandong First Medical University, Jinan 250014, Shandong, P. R. China

Abstract

We established an efficient method for single-cell miRNA analysis by droplet microfluidics, which has high sensitivity of single molecule detection and high throughput. Single-cell analysis of multiple miRNAs in various cells shows that miRNA expression is closely related to cancer type. CTC analysis shows that the method is applicable for rare cell analysis.

Chemical Communications

High-throughput and ultra-sensitive single-cell profiling of multiple microRNAs and identification of human cancer†

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High-throughput and ultra-sensitive single-cell profiling of multiple microRNAs and identification of human cancer

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