Pyrophosphate ion-responsive alginate hydrogel as an effective fluorescent sensing platform for alkaline phosphatase detection

Abstract

A pyrophosphate ion (PPi)-responsive alginate hydrogel was fabricated by using Cu²⁺ as a crosslinker. Benefiting from the ultrahigh affinity of Cu²⁺ to PPi, which can discriminate PPi from phosphate ions and other anions, the incorporation of carbon dots enables the alginate hydrogel to be an effective fluorescent sensing platform for the detection of alkaline phosphatase.