Issue 58, 2019

Branch migration based selective PCR for DNA mutation enrichment and detection

Abstract

We presented a branch migration based PCR in which a branch migration blocker was introduced to selectively reduce the amplification efficiency of the wild-type target and enrich the mutant-type target. The low-abundance mutations could be enriched and then detected by high resolution melting, Sanger sequencing or fluorescent DNA probe.

Graphical abstract: Branch migration based selective PCR for DNA mutation enrichment and detection

Supplementary files

Article information

Article type
Communication
Submitted
30 May 2019
Accepted
19 Jun 2019
First published
19 Jun 2019

Chem. Commun., 2019,55, 8466-8469

Branch migration based selective PCR for DNA mutation enrichment and detection

N. Chen, X. Ouyang, M. Lin, N. Liu, T. Wu and X. Xiao, Chem. Commun., 2019, 55, 8466 DOI: 10.1039/C9CC04161C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements