Issue 57, 2019

Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay

Abstract

Here we report a combined fluorescence and mass spectrometry assay which is capable of stably visualizing and quantifying cellular nucleoside-labeled RNA production and degradation. The fluorescence and mass spectrometry signals from cellular labeled RNAs show a linear correlation. This simple and robust assay benefits the biological community to study RNA metabolism.

Graphical abstract: Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay

Supplementary files

Article information

Article type
Communication
Submitted
21 May 2019
Accepted
20 Jun 2019
First published
21 Jun 2019

Chem. Commun., 2019,55, 8321-8324

Visualization and quantification of cellular RNA production and degradation using a combined fluorescence and mass spectrometry characterization assay

X. Gao, X. Shu, Y. Song, J. Cao, M. Gao, F. Wang, Y. Wang, J. Z. Sun, J. Liu and B. Z. Tang, Chem. Commun., 2019, 55, 8321 DOI: 10.1039/C9CC03923F

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