We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and “one-pot” biosensor for ultrasensitive detection of polynucleotide kinase (PNK). This method was also successfully applied in the screening of PNK inhibitors and analysis of the endogenous PNK activity at the single-cell level.
Y. Zhang, Y. Cui, X. Li, Y. Du, A. Tang and D. Kong, Chem. Commun., 2019, 55, 7611 DOI: 10.1039/C9CC03568K
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