Probing membrane enhanced protein–protein interactions in a minimal redox complex of cytochrome-P450 and P450-reductase

Mukesh Mahajan; Thirupathi Ravula; Elke Prade; G. M. Anantharamaiah; Ayyalusamy Ramamoorthy

doi:10.1039/C9CC01630A

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Probing membrane enhanced protein–protein interactions in a minimal redox complex of cytochrome-P450 and P450-reductase†

Mukesh Mahajan,

^a Thirupathi Ravula,

^a Elke Prade,^a G. M. Anantharamaiah^b and Ayyalusamy Ramamoorthy

*^a

Author affiliations

* Corresponding authors

^a Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA
E-mail: ramamoor@umich.edu

^b Department of Medicine, UAB Medical Center, Birmingham, Alabama 35294, USA

Abstract

Investigating the interplay in a minimal redox complex of cytochrome-P450 and its reductase is crucial for understanding cytochrome-P450's enzymatic activity. Probing the hotspots of dynamic structural interactions using NMR revealed the engagement of loop residues from P450-reductase to be responsible for the enhanced affinity of CYP450 towards its obligate redox partner.

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Article information

DOI: https://doi.org/10.1039/C9CC01630A
Article type: Communication
Submitted: 27 Feb 2019
Accepted: 17 Apr 2019
First published: 17 Apr 2019

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Chem. Commun., 2019,55, 5777-5780

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Probing membrane enhanced protein–protein interactions in a minimal redox complex of cytochrome-P450 and P450-reductase

M. Mahajan, T. Ravula, E. Prade, G. M. Anantharamaiah and A. Ramamoorthy, Chem. Commun., 2019, 55, 5777 DOI: 10.1039/C9CC01630A

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Chemical Communications

Probing membrane enhanced protein–protein interactions in a minimal redox complex of cytochrome-P450 and P450-reductase†

Abstract

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Probing membrane enhanced protein–protein interactions in a minimal redox complex of cytochrome-P450 and P450-reductase

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