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Abstract | Cited by

We report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, functions as a deep-red fluorescent indicator for the internal loop structure of the bacterial (Escherichia coli) ribosomal decoding region of the aminoacyl-tRNA site (A-site), which enables the assessment of A-site binding capability of various test compounds including blue and even-green-emitting compounds.

Graphical abstract: Trimethine cyanine dyes as deep-red fluorescent indicators with high selectivity to the internal loop of the bacterial A-site RNA

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