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Issue 31, 2019
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A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells

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Abstract

In this report, we designed and synthesized a novel fluorescent single tailed surfactant (termed FEDS), which can disrupt endosomes, complex lipofectamine, and can also identify cells that have been transfected. FEDS was able to increase the gene editing efficiency of lipofectamine/Cas9 ribonucleoprotein by 300% via a combination of fluorescent based enrichment and endosomal disruption.

Graphical abstract: A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells

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The article was received on 11 Jan 2019, accepted on 26 Mar 2019 and first published on 26 Mar 2019


Article type: Communication
DOI: 10.1039/C9CC00261H
Chem. Commun., 2019,55, 4562-4565

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    A novel fluorescent surfactant enhances the delivery of the Cas9 ribonucleoprotein and enables the identification of edited cells

    J. Li, J. J. Røise, J. Zhang, J. Yang, D. L. Kerr, H. Han and N. Murthy, Chem. Commun., 2019, 55, 4562
    DOI: 10.1039/C9CC00261H

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