Issue 27, 2019

Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

Abstract

A novel peroxidase-mimicking DNAzyme-based colorimetric homogenous bioassay is developed for kanamycin detection. Aptamer biorecognition triggers DNAzyme liberation from DNA duplexes for “off–on” signal transduction. The special hairpin structure of the aptamer–kanamycin biocomposite enables Exo III-assisted target recycling for signal amplification.

Graphical abstract: Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

Supplementary files

Article information

Article type
Communication
Submitted
21 Dec 2018
Accepted
01 Mar 2019
First published
02 Mar 2019

Chem. Commun., 2019,55, 3959-3962

Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

Z. Chen, F. Xiong, A. Yu and G. Lai, Chem. Commun., 2019, 55, 3959 DOI: 10.1039/C8CC10107H

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