Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

Zhichao Chen; Feng Xiong; Aimin Yu; Guosong Lai

doi:10.1039/C8CC10107H

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Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic†

Zhichao Chen,^a Feng Xiong,^a Aimin Yu

^ab and Guosong Lai

*^a

Author affiliations

* Corresponding authors

^a Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi 435002, P. R. China
E-mail: gslai@hbnu.edu.cn

^b Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn VIC 3122, Australia

Abstract

A novel peroxidase-mimicking DNAzyme-based colorimetric homogenous bioassay is developed for kanamycin detection. Aptamer biorecognition triggers DNAzyme liberation from DNA duplexes for “off–on” signal transduction. The special hairpin structure of the aptamer–kanamycin biocomposite enables Exo III-assisted target recycling for signal amplification.

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Article information

DOI: https://doi.org/10.1039/C8CC10107H
Article type: Communication
Submitted: 21 Dec 2018
Accepted: 01 Mar 2019
First published: 02 Mar 2019

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Chem. Commun., 2019,55, 3959-3962

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Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

Z. Chen, F. Xiong, A. Yu and G. Lai, Chem. Commun., 2019, 55, 3959 DOI: 10.1039/C8CC10107H

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Chemical Communications

Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic†

Abstract

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Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic

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