Issue 8, 2019
From the journal:

Chemical Communications

Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes†

Linghui Qian,*ab   Sijun Pan,a   Jun-Seok Lee, ORCID logo c   Jingyan Ge, ORCID logo d   Lin Li ORCID logo *e  and  Shao Q. Yao ORCID logo *a  

* Corresponding authors

a Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
E-mail: chmyaosq@nus.edu.sg

b Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
E-mail: lhqian@zju.edu.cn

c Molecular Recognition Research Center, Bio-Med Program of KIST-School UST, Korea Institute of Science & Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, South Korea

d Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China

e Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211800, China
E-mail: iamlli@njtech.edu.cn

Abstract

c-Jun N-terminal kinases (JNKs) are involved in critical cellular functions. Herein, small-molecule JNK-targeting probes are reported based on a covalent inhibitor. Together with newly developed two-photon fluorescence Turn-ON reporters and chemoproteomic studies, we showed that some probes may be suitable for live-cell imaging and profiling of JNKs.

Graphical abstract: Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes

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Article information

DOI
https://doi.org/10.1039/C8CC09558B
Article type
Communication
Submitted
01 Dec 2018
Accepted
21 Dec 2018
First published
26 Dec 2018

Chem. Commun., 2019,55, 1092-1095

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Live-cell imaging and profiling of c-Jun N-terminal kinases using covalent inhibitor-derived probes

L. Qian, S. Pan, J. Lee, J. Ge, L. Li and S. Q. Yao, Chem. Commun., 2019, 55, 1092 DOI: 10.1039/C8CC09558B

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