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Issue 23, 2019
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Development of a lateral flow immunoassay for the simultaneous detection of four dipyrone metabolites in milk

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Abstract

Dipyrone (DIP) is a commonly used non-steroidal anti-inflammatory drug with strong analgesic, antipyretic, and spasmolytic properties. Following oral administration, DIP is rapidly degraded into four major metabolites: 4-methylaminoantipyrine (MAA), 4-formylaminoantipyrine (FAA), 4-aminoantipyrine (AA), and 4-acetylaminoantipyrine (AAA). In this study, we produced a monoclonal antibody (mAb) and developed a lateral flow immunoassay (LFIA) for the simultaneous detection of the four DIP metabolites in milk samples. By comparing four haptens, a highly sensitive and specific mAb (3C7) was produced with 50% inhibitory concentrations of 8.67, 8.08, 0.98, and 0.82 ng mL−1 for AA, MAA, FAA, and AAA, respectively. Based on 3C7, an LFIA was successfully developed for the determination of DIP metabolites in spiked milk samples with visual limits of detection of 5, 5, 1, and 0.5 ng mL−1 for AA, MAA, FAA, and AAA, respectively, with recoveries (mean ± SD) ranging from 83.0 ± 3.3% to 103.8 ± 3.8%. Our findings revealed that the LFIA we developed is a potential method for the rapid and sensitive detection of DIP metabolites.

Graphical abstract: Development of a lateral flow immunoassay for the simultaneous detection of four dipyrone metabolites in milk

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Publication details

The article was received on 13 Apr 2019, accepted on 17 May 2019 and first published on 21 May 2019


Article type: Paper
DOI: 10.1039/C9AY00774A
Anal. Methods, 2019,11, 3041-3052

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    Development of a lateral flow immunoassay for the simultaneous detection of four dipyrone metabolites in milk

    Y. Li, L. Liu, H. Kuang and C. Xu, Anal. Methods, 2019, 11, 3041
    DOI: 10.1039/C9AY00774A

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