Issue 33, 2019

Investigation of cobalt bioaccumulation in Rhodopseudomonas palustris by proteomics analysis

Abstract

Cobalt bioaccumulation by Rhodopseudomonas palustris (R. palustris), which has different modes of metabolism for tolerating heavy metal stress, was investigated using proteomics analysis under aerobic-dark conditions in the presence of a high cobalt concentration. The metal tolerance stress of R. palustris was investigated and compared with the proteome of Co2+-exposed cells and cells grown in the control medium by two-dimensional (2-D) gel electrophoresis and mass spectrometry analysis. Significant changes in the expression level were detected for 36 proteins from the 2-D electrophoresis spectrum. Among them, 25 proteins were identified by MALDI TOF MS/MS, of which 19 were up-regulated and 6 were down-regulated, and they were divided into 7 categories based on their function, including protein synthesis, protein proteolysis, stress protein, carbohydrate metabolism, amino acid metabolism, transport protein and other proteins. The up-regulated mRNA expression of methionine adenosyltransferase (MAT) and phosphate ABC transporter substrate-binding protein (PSTS) was confirmed by semi-quantitative RT-PCR and quantitative RT PCR (qRT-PCR). The mRNA expression of Mat and Psts, which belong to the general category of amino acid metabolism for antioxidation and free phosphate radical transportation protein, increased 4.03-fold and 12.99-fold, respectively, in the process of cobalt bioaccumulation. These results indicate that the up-regulated MAT and PSTS are the key enzymes in the cobalt stress response in the metabolic process, and serve as a positive correlation between the content of the proteins and their mRNA levels.

Graphical abstract: Investigation of cobalt bioaccumulation in Rhodopseudomonas palustris by proteomics analysis

Article information

Article type
Paper
Submitted
25 Feb 2019
Accepted
23 Jul 2019
First published
06 Aug 2019

Anal. Methods, 2019,11, 4280-4288

Investigation of cobalt bioaccumulation in Rhodopseudomonas palustris by proteomics analysis

Y. Wang, C. Ma, J. Zhang, J. Tong, R. Gao and W. Dai, Anal. Methods, 2019, 11, 4280 DOI: 10.1039/C9AY00406H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements