Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 9, 2019
Previous Article Next Article

Amperometric enzyme sensor for the rapid determination of histamine

Author affiliations


The concentration of histamine, a biogenic amine, in fish is considered a fish spoilage indicator. Therefore, the development of a rapid-response and portable tool that allows its on-site analysis is very interesting since the consumption of high amounts of histamine causes scombroid poisoning. Thus, in this work a simple enzymatic sensor for the determination of histamine, based on a screen-printed carbon electrode and the enzyme diamine oxidase, was developed. The enzyme was immobilized on the electrode surface through a simple cross-linking procedure employing glutaraldehyde and bovine serum albumin. Chronoamperometry was used as the detection technique; the sensor showed a short response time (60 s, −0.3 V) and the measurements were performed by only using 40 μL of sample solution. The sensor provides a useful linear range (between 1 and 75 mg L−1 in fish extract), excellent reproducibility (RSD = 2.6%) and is reusable for up to 7 measurements. The feasibility of the sensor was tested performing histamine analysis in fish extracts achieving recovery values of 103%.

Graphical abstract: Amperometric enzyme sensor for the rapid determination of histamine

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Nov 2018, accepted on 06 Feb 2019 and first published on 08 Feb 2019

Article type: Paper
DOI: 10.1039/C8AY02610F
Citation: Anal. Methods, 2019,11, 1264-1269

  •   Request permissions

    Amperometric enzyme sensor for the rapid determination of histamine

    R. Torre, E. Costa-Rama, P. Lopes, H. P. A. Nouws and C. Delerue-Matos, Anal. Methods, 2019, 11, 1264
    DOI: 10.1039/C8AY02610F

Search articles by author