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Issue 9, 2019
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Amperometric enzyme sensor for the rapid determination of histamine

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Abstract

The concentration of histamine, a biogenic amine, in fish is considered a fish spoilage indicator. Therefore, the development of a rapid-response and portable tool that allows its on-site analysis is very interesting since the consumption of high amounts of histamine causes scombroid poisoning. Thus, in this work a simple enzymatic sensor for the determination of histamine, based on a screen-printed carbon electrode and the enzyme diamine oxidase, was developed. The enzyme was immobilized on the electrode surface through a simple cross-linking procedure employing glutaraldehyde and bovine serum albumin. Chronoamperometry was used as the detection technique; the sensor showed a short response time (60 s, −0.3 V) and the measurements were performed by only using 40 μL of sample solution. The sensor provides a useful linear range (between 1 and 75 mg L−1 in fish extract), excellent reproducibility (RSD = 2.6%) and is reusable for up to 7 measurements. The feasibility of the sensor was tested performing histamine analysis in fish extracts achieving recovery values of 103%.

Graphical abstract: Amperometric enzyme sensor for the rapid determination of histamine

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Publication details

The article was received on 29 Nov 2018, accepted on 06 Feb 2019 and first published on 08 Feb 2019


Article type: Paper
DOI: 10.1039/C8AY02610F
Citation: Anal. Methods, 2019,11, 1264-1269

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    Amperometric enzyme sensor for the rapid determination of histamine

    R. Torre, E. Costa-Rama, P. Lopes, H. P. A. Nouws and C. Delerue-Matos, Anal. Methods, 2019, 11, 1264
    DOI: 10.1039/C8AY02610F

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