Jump to main content
Jump to site search

Issue 15, 2019
Previous Article Next Article

An in cellulo-activated multicolor cell labeling approach used to image dying cell clearance

Author affiliations

Abstract

Dying cell clearance is critical for myriad biological processes such as tissue homeostasis. We herein report an enzyme-activated fluorescence cell labeling approach and its use for multicolor imaging of dying cell clearance. Diacetylated 4-hydroxymandelic acid (DHA)-conjugated dyes give rise to reactive quinone methides upon deacetylation in live cells, which in turn covalently labels cellular proteins. With partner cells tagged with distinct fluorescence, apoptotic cell clearance by Raw 264.7 macrophages and epithelial HeLa cells was captured by confocal microscopy, showing the potential of DHA-based cell labeling for investigating cell–cell interactions.

Graphical abstract: An in cellulo-activated multicolor cell labeling approach used to image dying cell clearance

Back to tab navigation

Supplementary files

Publication details

The article was received on 17 May 2019, accepted on 11 Jun 2019 and first published on 18 Jun 2019


Article type: Paper
DOI: 10.1039/C9AN00904C
Analyst, 2019,144, 4687-4693

  •   Request permissions

    An in cellulo-activated multicolor cell labeling approach used to image dying cell clearance

    Y. Shi, R. Zhu, Z. Xue, J. Han and S. Han, Analyst, 2019, 144, 4687
    DOI: 10.1039/C9AN00904C

Search articles by author

Spotlight

Advertisements