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Issue 5, 2019
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Rapid and sensitive detection of the activity of ADAM17 using a graphene oxide-based fluorescence sensor

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Abstract

A disintegrin and metalloproteinase 17 (ADAM17) has become a novel biomarker and potential therapeutic target for the early detection and treatment of human cancers. In this work, by covalently attaching fluorescently labeled ADAM17 substrate peptide (Pep-FAM) molecules to carboxylated graphene oxide (cGO) and monitoring the cleavage of the peptide substrate by ADAM17, we developed a cGO-Pep-FAM fluorescence sensor for the rapid, sensitive and accurate detection of ADAM17. The sensor was highly sensitive with a detection limit of 17.5 picomolar. Furthermore, the sensor was selective: structure similar proteases such as ADAM9 and MMP-9 would not interfere with ADAM17 detection. In addition, simulated serum samples were successfully analyzed. Our developed cGO-Pep-FAM sensing strategy should find useful applications in disease diagnosis and drug screening.

Graphical abstract: Rapid and sensitive detection of the activity of ADAM17 using a graphene oxide-based fluorescence sensor

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Supplementary files

Article information


Submitted
03 Dec 2018
Accepted
09 Jan 2019
First published
11 Jan 2019

Analyst, 2019,144, 1825-1830
Article type
Paper
Author version available

Rapid and sensitive detection of the activity of ADAM17 using a graphene oxide-based fluorescence sensor

Y. Zhang, X. Chen, G. M. Roozbahani and X. Guan, Analyst, 2019, 144, 1825
DOI: 10.1039/C8AN02344A

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