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Issue 9, 2019
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A pH-based sensor array for the detection and identification of proteins using CdSe/ZnS quantum dots as an indicator

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Abstract

Protein identification is very important in the field of clinical medicine and diagnosis. Here, we report a novel and simple sensor array for the detection and identification of proteins using pH buffer solutions as sensing elements. Different proteins in various pH solutions have different net surface charges including positive, negative or no charge. Such differences may allow a pattern recognition-based sensor array for protein identification. When using negatively charged CdSe/ZnS quantum dots as an indicator, the interactions between a charged protein and quantum dots result in fluorescence changes, generating a differential response pattern for the protein. The result shows that proteins with pI > 7, pI = 7 or pI < 7 can be differentiated successfully. Moreover, complex protein mixtures are also able to be identified and the results demonstrate that surface charge may play an important role in protein sensing. The HSA of different concentrations in water and human urine can also be detected by using the sensor array. It is demonstrated that the proposed sensor array has potential applications in clinical diagnosis and proteomics.

Graphical abstract: A pH-based sensor array for the detection and identification of proteins using CdSe/ZnS quantum dots as an indicator

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Publication details

The article was received on 24 Nov 2018, accepted on 19 Feb 2019 and first published on 22 Feb 2019


Article type: Paper
DOI: 10.1039/C8AN02285B
Analyst, 2019,144, 2891-2897

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    A pH-based sensor array for the detection and identification of proteins using CdSe/ZnS quantum dots as an indicator

    P. Yan, X. Li, Y. Dong, B. Li and Y. Wu, Analyst, 2019, 144, 2891
    DOI: 10.1039/C8AN02285B

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