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Near-infrared mito-specific fluorescent probe for ratiometric detection and imaging of alkaline phosphatase activity with high sensitivity

Abstract

Fluorescent probe-based analytical method for biological species has gained increasing attention for its powerful detection and imaging capabilities. However, sometimes it is still problematic for accurate analyses because it can be influenced by the probe concentration, intrinsic fluorescence, instrumental factors or environmental conditions and intense laser irradiation is usually harmful to biological systems. A NIR ratiometric fluorescent probe can eliminate the influence of these factors and lead to a higher sensing performance. We propose to introduce a reasonable masking group onto the meso oxygen atom of a cyanine framework (Cy-O) to achieve a NIR ratiometric probe by modulating the conjugated polymethine π-electron system of cyanine dye. Reported here is our proof-of-the-concept attempt for a highly sensitive and selective mito-specific NIR ratiometric fluorescent sensing strategy for ALP, and phosphate group was chosen as a functional group for ALP catalyzed hydrolysis. Upon treated with ALP, the probe Cy-OP exhibits excellent recognition properties such as high selectivity, high sensitivity (with a low detection limit of 0.16 mU/mL), short response time (within 30 min) and a large spectral shift (from 736 to 516 nm in absorption spectra and 766 to 616 nm in emission spectra) with low fluorescence background. Moreover, the ratio of emission (I 616 nm/I 766 nm) of the enzymatic residue was favorable for its application in complex bio-system. Consequently, the well-designed probe was successfully applied in detection of ALP in living cells with satisfactory results.

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Publication details

The article was received on 24 Oct 2018, accepted on 03 Dec 2018 and first published on 06 Dec 2018


Article type: Paper
DOI: 10.1039/C8TB02799D
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Near-infrared mito-specific fluorescent probe for ratiometric detection and imaging of alkaline phosphatase activity with high sensitivity

    Q. Zhang, S. Li, C. Fu, Y. Xiao, P. Zhang and C. Ding, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB02799D

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