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Peroxidase-like activity of acetylcholine based colorimetric detection of acetylcholinesterase activity and inhibitor organophosphorus

Abstract

Colorimetric detection of acetylcholinesterase (AChE) and its inhibitor organophosphates (OPs) is attractive for its convenience, but the extra addition of exogenous catalyst to produce the chromogenic agent may result in the complexity and interference. Herein, we firstly found acetylcholine (ATCh) itself possessed the mimic peroxidase’s activity, based on which, a simple and reliable colorimetric system containing ATCh- 3, 3, 5, 5 - tetramethylbenzidine (TMB)-H2O2 was developed for the sensitive and selective assay of AChE activity and futher its inhibitor OPs. Due to the AChE catalyzed hydrolysis of acetylcholine, the mimic peroxidase activity was affected, which was used for the highly detection of AChE activity with a low limit of detection (LOD) as 0.5 mU/mL and a linear range from 2.0 to 14 mU/mL. Furthermore, by the inhibition of OPs on the AChE, OPs were detected with the present ATCh regulated colorimetric system with the LOD of 4.0 ng/mL, and linear range from 10 to 10000 μg/L. This strategy was also demonstrated to be applicable for pesticide detection in real samples. Meanwhile the sensing platform can also be implemented on test strips for rapid and visual monitoring of OPs. Thus, this extremely simple colorimetric strategy without the addtion of other exogenous catalyst holds great promise for pesticide detection on-site and can be further exploited for sensing applications in the environment and the food safety field.

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Publication details

The article was received on 04 Oct 2018, accepted on 27 Nov 2018 and first published on 29 Nov 2018


Article type: Paper
DOI: 10.1039/C8TB02616E
Citation: J. Mater. Chem. B, 2018, Accepted Manuscript
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    Peroxidase-like activity of acetylcholine based colorimetric detection of acetylcholinesterase activity and inhibitor organophosphorus

    T. Han and G. Wang, J. Mater. Chem. B, 2018, Accepted Manuscript , DOI: 10.1039/C8TB02616E

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