Issue 46, 2018

Enzymatically crosslinked poly(2-alkyl-2-oxazoline) networks for 3D cell culture

Abstract

Molecularly designed, random copolymer-bioconjugates based on poly(2-methyl-2-oxazoline) (PMOXA) and poly(2-ethyl-2-oxazoline) (PEOXA) are crosslinked in the presence of sortase A (SA) and human articular chondrocytes (hACs), to yield cellularized polymer networks. Their gelation kinetics and mechanical properties are finely tuned by varying the concentration of SA, while a cell viability >90% is achieved after several weeks of culture, even in the absence of any cell-adhesive cue.

Graphical abstract: Enzymatically crosslinked poly(2-alkyl-2-oxazoline) networks for 3D cell culture

Supplementary files

Article information

Article type
Communication
Submitted
10 Sep 2018
Accepted
20 Oct 2018
First published
22 Oct 2018

J. Mater. Chem. B, 2018,6, 7568-7572

Enzymatically crosslinked poly(2-alkyl-2-oxazoline) networks for 3D cell culture

L. Trachsel, N. Broguiere, J. Rosenboom, M. Zenobi-Wong and E. M. Benetti, J. Mater. Chem. B, 2018, 6, 7568 DOI: 10.1039/C8TB02382D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements