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Issue 42, 2018
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Facile fabrication of a “Catch and Release” cellulose acetate nanofiber interface: a platform for reversible glycoprotein capture and bacterial attachment

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Abstract

Surfaces engineered to selectively capture and release target biomolecules and cells “on demand” are of considerable interest in a variety of biomedical applications, such as diagnostics and detection. In this work, a strategy is proposed to achieve “catch and release” using boronic acid ligand-functionalized electrospun cellulose acetate (CA) nanofiber mats. To achieve this goal, a simple method based on the one-step visible light-induced grafting copolymerization of 3-(acrylamido)phenylboronic acid (BA) and acrylamide (AM) is established. The copolymer poly(AM-co-BA)-grafted CA nanofiber mats (CA-g-P(AM-co-BA)) showed high capacity for the binding of glycoproteins such as horseradish peroxidase (HRP) and ovalbumin (OVA) under alkaline (pH 9.0) and physiological conditions (pH 7.4). Moreover, the bound glycoproteins can be released from the CA-g-P(AM-co-BA) mats by simple exposure to an acetic acid–sodium acetate (HAc–NaAc) buffer solution (pH 4.0). In addition, this system can be applied to directly extract glycoproteins from egg white samples. Likewise, the CA-g-P(AM-co-BA) mats acted as favorable substrates for Gram-negative E. coli bacterial attachment. The release of the attached bacteria is triggered by competitive sugar binding involving the addition of glucose. It can be envisioned that such boronic acid ligand-functionalized CA nanofiber mats that can “catch and release” biomolecules and bacterial cells hold considerable promise for diverse applications.

Graphical abstract: Facile fabrication of a “Catch and Release” cellulose acetate nanofiber interface: a platform for reversible glycoprotein capture and bacterial attachment

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Publication details

The article was received on 31 Aug 2018, accepted on 28 Sep 2018 and first published on 03 Oct 2018


Article type: Paper
DOI: 10.1039/C8TB02291G
Citation: J. Mater. Chem. B, 2018,6, 6744-6751
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    Facile fabrication of a “Catch and Release” cellulose acetate nanofiber interface: a platform for reversible glycoprotein capture and bacterial attachment

    Y. Dong, X. Lu, P. Wang, W. Liu, S. Zhang, Z. Wu and H. Chen, J. Mater. Chem. B, 2018, 6, 6744
    DOI: 10.1039/C8TB02291G

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