Jump to main content
Jump to site search


Dual stimuli-responsive saccharide core based nanocarrier for efficient Birc5-shRNA delivery

Author affiliations

Abstract

Stimuli-responsive delivery systems show great promise in meeting the requirements of several delivery stages to achieve satisfactory gene transfection. This report describes a novel stimuli-responsive nanocarrier consisting of a dual stimuli-responsive chitosan (SCS) core and a polyethyleneimine (PEI) and polyethylene glycol (PEG) corona. The SCS core was constructed by cross-linking bio-safe chitosan saccharide via pH-sensitive hydrazone bonds and redox-sensitive disulfide bonds, which conferred the nanocarrier with a dual stimuli-responsive property. PEG and low-molecular-weight PEI were further immobilized on the core to endow the synthesized SCS-g-PEI-g-PEG with improved gene condensation, prolonged circulation and enhanced lysosome escape. In vitro and in vivo Birc5-shRNA delivery results revealed that significantly enhanced gene expression and tumor inhibition were achieved by SCS-g-PEI-g-PEG, compared with the non-stimuli-responsive counterpart CS-g-PEI-g-PEG. Moreover, the SCS-g-PEI-g-PEG nanocarrier was biocompatible as shown in both in vitro and in vivo toxicity evaluations. This study may provide a promising strategy for constructing an efficient and bio-safe delivery system for gene therapy.

Graphical abstract: Dual stimuli-responsive saccharide core based nanocarrier for efficient Birc5-shRNA delivery

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Jun 2018, accepted on 18 Oct 2018 and first published on 18 Oct 2018


Article type: Paper
DOI: 10.1039/C8TB01683F
Citation: J. Mater. Chem. B, 2018, Advance Article
  •   Request permissions

    Dual stimuli-responsive saccharide core based nanocarrier for efficient Birc5-shRNA delivery

    H. Chen, Y. Ma, H. Lan, Y. Zhao, D. Zhi, S. Cui, J. Du, Z. Zhang, Y. Zhen and S. Zhang, J. Mater. Chem. B, 2018, Advance Article , DOI: 10.1039/C8TB01683F

Search articles by author

Spotlight

Advertisements