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Issue 42, 2018
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Polycaprolactone nanofibers functionalized with placental derived extracellular matrix for stimulating wound healing activity

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Abstract

Impaired wound healing is primarily associated with inadequate angiogenesis, repressed cell migration, deficient synthesis of extracellular matrix (ECM) component/growth factors, and altered inflammatory responses in the wound bed environment. Herein, we report a simple process for the fabrication of PCL nanofiber mats embedded with placental-derived bioactive molecules (PCL–sPEM) rich in growth factors for full-thickness cutaneous wound healing. The physicochemical attributes and biological composition of PCL–sPEM nanofiber mats delivered a nontoxic environment in vitro and significantly promoted the adhesion, infiltration, and proliferation of human fibroblasts/keratinocytes. Conditioned media extracted from PCL–sPEM nanofiber mats enhanced the migration potential of the cells (fibroblasts/keratinocytes) involved in wound healing due to the release of growth factors embedded in it. Further, PCL–sPEM nanofiber mats attracted, stimulated and supported vascularization as determined by the Chick Chorioallantoic Membrane (CAM) assay. Interestingly, critical skin wounds of rats treated with PCL–sPEM nanofiber mats facilitated improved wound closure with well-organized dermis and epidermis, which could be ascribed to prominent vascularization, augmented migration of human foreskin fibroblasts (HFFs) & human epidermal keratinocytes (HEKs), increased collagen synthesis and early re-epithelialization. Collectively, our results suggest that PCL–sPEM nanofiber mats embedded with growth factors could be a suitable matrix for treating critical full-thickness wounds.

Graphical abstract: Polycaprolactone nanofibers functionalized with placental derived extracellular matrix for stimulating wound healing activity

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Publication details

The article was received on 24 May 2018, accepted on 02 Oct 2018 and first published on 03 Oct 2018


Article type: Paper
DOI: 10.1039/C8TB01373J
Citation: J. Mater. Chem. B, 2018,6, 6767-6780
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    Polycaprolactone nanofibers functionalized with placental derived extracellular matrix for stimulating wound healing activity

    A. P. Rameshbabu, S. Datta, K. Bankoti, E. Subramani, K. Chaudhury, V. Lalzawmliana, S. K. Nandi and S. Dhara, J. Mater. Chem. B, 2018, 6, 6767
    DOI: 10.1039/C8TB01373J

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