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Issue 43, 2018
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New class of artificial enzyme composed of Mn-porphyrin, imidazole, and cucurbit[10]uril toward use as a therapeutic antioxidant

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Abstract

In this study, we investigated a new class of artificial enzymes composed of Mn-porphyrin, imidazole, and cucurbit[10]uril (CB[10]) toward therapeutic antioxidants. Structural characterization by means of NMR indicated that the inclusion mode of metalloporphyrin in CB[10] was sensitive to the chemical structure of metalloporphyrin and that the structure of the artificial enzyme had a similarly to that of native heme catalase. Kinetic analysis for catalytic antioxidative activities demonstrated that the artificial enzyme exhibited highly efficient activity for H2O2 disproportionation (catalase activity) in water. The activity was classified as top-performing among the water-soluble artificial catalases. The artificial enzyme was constructed by simply mixing the components in water. We consider that this is a great advantage over previously reported artificial catalases, which require a multi-step synthesis or that lack water solubility. The pro-oxidative peroxidase activity was remarkably suppressed due to inclusion in CB[10]. Furthermore, a preliminary in vitro study suggested that the artificial enzyme catalytically eliminated reactive oxygen species, including H2O2, in human cell lines. It was presumed that CB[10] contributed to the bioavailability of the artificial enzyme. Overall, the artificial enzyme was shown to have high potential as a therapeutic antioxidant. We consider that the results in this study could lead to a new conceptual advance toward therapeutic antioxidants that could simultaneously improve the catalytic and biological properties of Mn-porphyrins.

Graphical abstract: New class of artificial enzyme composed of Mn-porphyrin, imidazole, and cucurbit[10]uril toward use as a therapeutic antioxidant

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Publication details

The article was received on 07 May 2018, accepted on 03 Oct 2018 and first published on 19 Oct 2018


Article type: Paper
DOI: 10.1039/C8TB01204K
Citation: J. Mater. Chem. B, 2018,6, 7050-7059
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    New class of artificial enzyme composed of Mn-porphyrin, imidazole, and cucurbit[10]uril toward use as a therapeutic antioxidant

    R. Kubota, T. Takabe, K. Arima, H. Taniguchi, S. Asayama and H. Kawakami, J. Mater. Chem. B, 2018, 6, 7050
    DOI: 10.1039/C8TB01204K

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