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Bridging the spatiotemporal scales of macromolecular transport in crowded biomimetic systems

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Abstract

Crowding plays a key role in the transport and conformations of biological macromolecules. Gene therapy, viral infection, and transfection require DNA to traverse the crowded cytoplasm, including the cytoskeletal network of filamentous proteins. Given the complexity of cellular crowding, the dynamics of biological molecules can be highly dependent on the spatiotemporal scale probed. We present a powerful platform that spans molecular and cellular scales by coupling single-molecule conformational tracking (SMCT) and selective-plane illumination differential dynamic microscopy (SPIDDM). We elucidate the transport and conformational properties of large DNA, crowded by custom-designed networks of actin and microtubules, to link single-molecule conformations with ensemble DNA transport and cytoskeleton structure. We show that actin crowding leads to DNA compaction and suppression of fluctuations, combined with subdiffusion and heterogeneous transport, whereas microtubules have much more subdued impact across all scales. In composite networks of both filaments, scale-dependent effects emerge such that actin dictates ensemble DNA transport while microtubules influence single-molecule dynamics. We show that these intriguing results arise from a complex interplay between network rigidity, mesh size, filament concentration, and DNA size.

Graphical abstract: Bridging the spatiotemporal scales of macromolecular transport in crowded biomimetic systems

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Publication details

The article was received on 04 Oct 2018, accepted on 05 Dec 2018 and first published on 05 Dec 2018


Article type: Communication
DOI: 10.1039/C8SM02023J
Citation: Soft Matter, 2019, Advance Article
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    Bridging the spatiotemporal scales of macromolecular transport in crowded biomimetic systems

    K. Regan, D. Wulstein, H. Rasmussen, R. McGorty and R. M. Robertson-Anderson, Soft Matter, 2019, Advance Article , DOI: 10.1039/C8SM02023J

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