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Issue 47, 2018
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Single-stranded DNA oligomer brush structure is dominated by intramolecular interactions mediated by the ion environment

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Abstract

Single-stranded DNA (ssDNA) brushes, in which ssDNA oligomers are tethered to surfaces in dense monolayers, are being investigated for potential biosensing applications. The structure of the brush can affect the selectivity and the hybridization efficiency of the device. The structure is commonly thought to result from the balance of intramolecular interactions, intermolecular interactions within the monolayer, and molecule–surface interactions. Here, we test the hypothesis that ssDNA oligomer brush structure is dominated by intramolecular interactions. We use AFM to measure the height of an ssDNA brush and molecular dynamics to simulate the end-to-end distance, both as a function of ionic strength of the surrounding solution. The brush height and the molecule end-to-end distance match quantitatively, providing evidence that the brush structure is dominated by intramolecular interactions (mediated by ions). The physical basis of the intramolecular interactions is elucidated by the simulations.

Graphical abstract: Single-stranded DNA oligomer brush structure is dominated by intramolecular interactions mediated by the ion environment

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Publication details

The article was received on 24 Aug 2018, accepted on 15 Nov 2018 and first published on 16 Nov 2018


Article type: Paper
DOI: 10.1039/C8SM01743C
Citation: Soft Matter, 2018,14, 9675-9680
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    Single-stranded DNA oligomer brush structure is dominated by intramolecular interactions mediated by the ion environment

    P. S. Gil, D. J. Lacks, P. Parisse, L. Casalis and M. D. Nkoua Ngavouka, Soft Matter, 2018, 14, 9675
    DOI: 10.1039/C8SM01743C

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