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Issue 47, 2018
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The Alzheimer's disease amyloid-β peptide affects the size-dynamics of raft-mimicking Lo domains in GM1-containing lipid bilayers

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Abstract

Alzheimer's disease (AD) is characterized by the overproduction of the amyloid-β peptide (Aβ) which forms fibrils under the influence of raft microdomains containing the ganglioside GM1. Raft-mimicking artificial liquid ordered (Lo) domains containing GM1 enhance amyloid-β polymerization. Other experiments suggest that Aβ binds preferably to the non-raft liquid disordered (Ld) phase rather than to the Lo phase in the presence of GM1. Here, the interaction of Aβ(1–42) with GM1-containing biphasic Lo–Ld giant vesicles was investigated. Fluorescence colocalisation experiments confirm that Aβ(1–42) binds preferentially to the Ld phase. The effect of Aβ(1–42) on the Lo–Ld size dynamics was studied using photoinduced spinodal decomposition which mimics the nanodomain–microdomain raft coalescence. Aβ affects the kinetics of the coarsening phase and the size of the resulting microdomains. The effect depends on which phase is in a majority: when the Lo microdomains are formed inside an Ld phase, their growth rate becomes slower and their final size smaller in the presence of Aβ(1–42), whereas when the Ld microdomains are formed inside an Lo phase, the growth rate becomes faster and the final size larger. Fluorimetric measurements on large vesicles using the probe Laurdan indicate that Aβ(1–42) binding respectively increases or decreases the packing of the Ld phase in the presence or absence of GM1. The differential effects of Aβ on spinodal decomposition are accordingly interpreted as resulting from distinct effects of the peptide on the Lo–Ld line tension modulated by GM1. Such modulating effect of Aβ on domain dynamics could be important for lipid rafts in signaling disorders in AD as well as in Aβ fibrillation.

Graphical abstract: The Alzheimer's disease amyloid-β peptide affects the size-dynamics of raft-mimicking Lo domains in GM1-containing lipid bilayers

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Publication details

The article was received on 09 Aug 2018, accepted on 08 Nov 2018 and first published on 12 Nov 2018


Article type: Paper
DOI: 10.1039/C8SM01636D
Citation: Soft Matter, 2018,14, 9609-9618
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    The Alzheimer's disease amyloid-β peptide affects the size-dynamics of raft-mimicking Lo domains in GM1-containing lipid bilayers

    G. Staneva, N. Puff, S. Stanimirov, T. Tochev, M. I. Angelova and M. Seigneuret, Soft Matter, 2018, 14, 9609
    DOI: 10.1039/C8SM01636D

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