Capillary flow and mechanical buckling in a growing annular bacterial colony

Abstract

A growing bacterial colony is a dense suspension of an increasing number of cells capable of individual as well as collective motion. After inoculating Pseudomonas aeruginosa over an annular area on an agar plate, we observe the growth and spread of the bacterial population, and model the process by considering the physical effects that account for the features observed. Over a course of 10–12 hours, the majority of bacteria migrate to and accumulate at the edges. We model the capillary flow induced by imbalanced evaporation flux as the cause for the accumulation, much like the well-known coffee stain phenomenon. Simultaneously, periodic buckles or protrusions occur at the inner edge. These buckles indicate that the crowding bacteria produce a jam, transforming the densely packed population at the inner edge to a solid state. The continued bacterial growth produces buckles. Subsequently, a ring of packed bacteria behind the inner edge detach from it and break into pieces, forming bacterial droplets. These droplets slowly coalesce while they continually grow and collectively surf on the agar surface in the region where the colony had previously spread over. Our study shows a clear example of how fluid dynamics and elasto-mechanics together govern the bacterial colony pattern evolution.