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Issue 27, 2018
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Combined scanning electrochemical and fluorescence microscopies using a tetrazine as a single redox and luminescent (electrofluorochromic) probe

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Abstract

The possibility of using a single electroactive and luminescent molecule both as a redox mediator and as a fluorophore in an experiment combining in situ Scanning Electrochemical Microscopy (SECM) and epifluorescence microscopy was validated. The usual working modes of SECM, namely positive and negative feedback as well as generation–collection, were used and the fluorescence images, intensity and spectra were recorded for each configuration. The tip potential, tip–substrate distance and, in the case of a conducting substrate, the substrate potential are the parameters that are likely to control the fluorescence. It is shown that the tip can be used to switch on and off the luminescence and that the modulation amplitude maximum is sensitive to the nature of the substrate. Approach curves based on this fluorescence modulation amplitude can be obtained showing a higher sensitivity than the classical electrochemical ones.

Graphical abstract: Combined scanning electrochemical and fluorescence microscopies using a tetrazine as a single redox and luminescent (electrofluorochromic) probe

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Supplementary files

Article information


Submitted
20 Apr 2018
Accepted
29 May 2018
First published
30 May 2018

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2018,9, 5897-5905
Article type
Edge Article

Combined scanning electrochemical and fluorescence microscopies using a tetrazine as a single redox and luminescent (electrofluorochromic) probe

L. Guerret-Legras, J. F. Audibert, G. V. Dubacheva and F. Miomandre, Chem. Sci., 2018, 9, 5897
DOI: 10.1039/C8SC01814F

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