Issue 18, 2018

Protein surface functionalisation as a general strategy for facilitating biomimetic mineralisation of ZIF-8

Abstract

The durability of enzymes in harsh conditions can be enhanced by encapsulation within metal–organic frameworks (MOFs) via a process called biomimetic mineralisation. Herein we show that the surface charge and chemistry of a protein determines its ability to seed MOF growth. We demonstrate that chemical modification of amino acids on the protein surface is an effective method for systematically controlling biomimetic mineralisation by zeolitic imidazolate framework-8 (ZIF-8). Reaction of surface lysine residues with succinic (or acetic) anhydride facilitates biomimetic mineralisation by increasing the surface negative charge, whereas reaction of surface carboxylate moieties with ethylenediamine affords a more positively charged protein and hinders the process. Moreover, computational studies confirm that the surface electrostatic potential of a protein is a good indicator of its ability to induce biomimetic mineralisation. This study highlights the important role played by protein surface chemistry in encapsulation and outlines a general method for facilitating the biomimetic mineralisation of proteins.

Graphical abstract: Protein surface functionalisation as a general strategy for facilitating biomimetic mineralisation of ZIF-8

Supplementary files

Article information

Article type
Edge Article
Submitted
20 Feb 2018
Accepted
09 Mar 2018
First published
09 Mar 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2018,9, 4217-4223

Protein surface functionalisation as a general strategy for facilitating biomimetic mineralisation of ZIF-8

N. K. Maddigan, A. Tarzia, D. M. Huang, C. J. Sumby, S. G. Bell, P. Falcaro and Christian. J. Doonan, Chem. Sci., 2018, 9, 4217 DOI: 10.1039/C8SC00825F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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