Jump to main content
Jump to site search

Issue 48, 2018, Issue in Progress
Previous Article Next Article

One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging

Author affiliations

Abstract

The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery. Their successful conjugation to biocompatible agents is crucial for specific molecules recognition and usually requires multiple steps which may lead to low reproducibility. Here, we report a simple and rapid one-step procedure for in situ synthesis of biocompatible amino-functionalized NaYF4:Yb,Er UCNPs that could be used for NIR-driven fluorescence cell labeling. X-ray diffraction showed that UCNPs synthesized through chitosan-assisted solvothermal processing are monophasic and crystallize in a cubic α phase. Scanning and transmission electron microscopy revealed that the obtained crystals are spherical in shape with a mean diameter of 120 nm. Photoluminescence spectra indicated weaker green (2H11/2, 4S3/24I15/2) and stronger red emission (4F9/24I15/2), as a result of enhanced non-radiative 4I11/24I13/2 Er3+ relaxation. The presence of chitosan groups at the surface of UCNPs was confirmed by Fourier transform infrared spectroscopy, thermogravimetry and X-ray photoelectron spectroscopy. This provides their enhanced internalization in cells, at low concentration of 10 μg ml−1, without suppression of cell viability after 24 h of exposure. Furthermore, upon 980 nm laser irradiation, the amino-functionalized NaYF4:Yb,Er UCNPs were successfully used in vitro for labeling of two human cell types, normal gingival and oral squamous cell carcinoma.

Graphical abstract: One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 May 2018, accepted on 25 Jul 2018 and first published on 01 Aug 2018


Article type: Paper
DOI: 10.1039/C8RA04178D
Citation: RSC Adv., 2018,8, 27429-27437
  • Open access: Creative Commons BY license
  •   Request permissions

    One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging

    L. Mancic, A. Djukic-Vukovic, I. Dinic, M. G. Nikolic, M. D. Rabasovic, A. J. Krmpot, A. M. L. M. Costa, B. A. Marinkovic, L. Mojovic and O. Milosevic, RSC Adv., 2018, 8, 27429
    DOI: 10.1039/C8RA04178D

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements