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Issue 42, 2018, Issue in Progress
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A multifunctional oxidosqualene cyclase from Tripterygium regelii that produces both α- and β-amyrin

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Abstract

Tripterygium regelii is a rich source of triterpenoids, containing many types of triterpenes with high chemical diversity and interesting pharmacological properties. The cDNA of the multifunctional oxidosqualene cyclase (TrOSC, GenBank accession number: MH161182), consisting of a 2289 bp open reading frame and coding for 762 amino acids, was cloned from the stems and roots of Tripterygium regelii. Phylogenetic analysis using OSC genes from other plants suggested that TrOSC might be a mixed-amyrin synthase. The coding sequence was cloned into the expression vector pYES2 and transformed into the yeast Saccharomyces cerevisiae. The resulting products were analysed by GC-MS. Surprisingly, although it showed 76% sequence identity to lupeol synthase from Ricinus communis, TrOSC was found to be a multifunctional triterpene synthase producing both α- and β-amyrin, the precursors of ursane and oleanane type triterpenes, respectively. qRT-PCR analysis revealed that the transcript of TrOSC accumulated mainly in roots and stems. Taken together, our findings contribute to the knowledge of key genes in the pentacyclic triterpene biosynthesis pathway.

Graphical abstract: A multifunctional oxidosqualene cyclase from Tripterygium regelii that produces both α- and β-amyrin

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Supplementary files

Article information


Submitted
23 Apr 2018
Accepted
16 Jun 2018
First published
27 Jun 2018

This article is Open Access

RSC Adv., 2018,8, 23516-23521
Article type
Paper

A multifunctional oxidosqualene cyclase from Tripterygium regelii that produces both α- and β-amyrin

Y. Lu, J. Zhou, T. Hu, Y. Zhang, P. Su, J. Wang, W. Gao and L. Huang, RSC Adv., 2018, 8, 23516
DOI: 10.1039/C8RA03468K

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