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Rational design of a ratiometric and lysosome-targetable AIE dots for imaging endogenous HClO in live cells

Abstract

Rational design of novel ratiometric and targetable fluorescent probes for selective imaging of lysosomal hypochlorous acid (HClO) is conducive to investigate the function of HClO in pathophysiological process. Herein, we for the first time combine 1-pyrenecarboxaldehyde (with π-π stacking effect) and 2-methylquinoline derivatives to synthesize an aggregation-induced-emission (AIE) probe (SA-C2-PCD) for HClO ratiometric detection in living cells. Subsequently, a kind of novel lysosome-targetable AIE dots (AIED-Lys) with dual emission feature was prepared via a simple co-precipitation of lysosome-targeting surfactant MAA-CO720, amphiphilic block copolymers PEO113-b-PS46 and HClO-responsive probe (SA-C2-PCD). The free AIED-Lys shows only strong red AIE fluorescence without addition of HClO. However, when exposed to the strong oxidizing HClO, the SA-C2-PCD would release 1-pyrenecarboxaldehyde that aggregates through π-π stack interaction in aqueous solutions, inducing a distinct fluorescence change from red to blue. Interestingly, the as-prepared AIED-Lys shows good water dispersity, high selectivity and sensitivity (~ 70.4 nM), strong anti-interference ability, prominent long-term fluorescence stability (>10 weeks), and low cytotoxicity, and can be successfully utilized to visually detecting the endogenous HClO in lysosomes in live cells.

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Publication details

The article was received on 08 Oct 2018, accepted on 30 Nov 2018 and first published on 04 Dec 2018


Article type: Research Article
DOI: 10.1039/C8QM00511G
Citation: Mater. Chem. Front., 2018, Accepted Manuscript
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    Rational design of a ratiometric and lysosome-targetable AIE dots for imaging endogenous HClO in live cells

    X. Yong, H. Wang, M. Xue, P. Zhang, W. Liu, S. Chen, R. Zeng, J. Cui, Y. Gao and J. Chen, Mater. Chem. Front., 2018, Accepted Manuscript , DOI: 10.1039/C8QM00511G

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