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Issue 6, 2018
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Selection and characterization of a DNA aptamer to crystal violet

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Abstract

Aptamers are short single-stranded DNA or RNA, which can be selected in vitro by systematic evolution of ligands by exponential enrichment (SELEX). In order to develop novel light-up probes to substitute G-quadruplex (G4), we selected a DNA aptamer for crystal violet (CV), a triphenylmethane light-up dye, by a modified affinity chromatography-based SELEX. The ssDNA pool was first coupled on streptavidin-coated agarose beads through a biotin labeled complementary oligonucleotide, and then the aptamer sequences would be released from agarose beads by CV affinity. This method is simple, straightforward and effective. The aptamer sequence with a low micromolar dissociation constant (Kd) and good specificity was achieved after 11 rounds of selection. The light-up properties of the CV–aptamer were also investigated, and the CV showed dramatic fluorescence enhancement. The CV–aptamer pair could be further used as a novel light-up fluorescent probe to design biosensors.

Graphical abstract: Selection and characterization of a DNA aptamer to crystal violet

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Publication details

The article was received on 20 Dec 2017, accepted on 23 Apr 2018 and first published on 25 Apr 2018


Article type: Paper
DOI: 10.1039/C7PP00457E
Citation: Photochem. Photobiol. Sci., 2018,17, 800-806
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    Selection and characterization of a DNA aptamer to crystal violet

    Y. Chen, J. Wang, Y. Zhang, L. Xu, T. Gao, B. Wang and R. Pei, Photochem. Photobiol. Sci., 2018, 17, 800
    DOI: 10.1039/C7PP00457E

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