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Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA

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Abstract

A prominent hurdle in developing small molecule probes against RNA is the relative scarcity of general screening methods. In this study, we demonstrate the application of a fluorescent peptide displacement assay to screen small molecule probes against four different RNA targets. The designed experimental protocol combined with statistical analysis provides a fast and convenient method to simultaneously evaluate small molecule libraries against different RNA targets and classify them based on affinity and selectivity patterns.

Graphical abstract: Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA

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Publication details

The article was received on 04 Oct 2018, accepted on 19 Nov 2018 and first published on 20 Nov 2018


Article type: Communication
DOI: 10.1039/C8OB02467G
Citation: Org. Biomol. Chem., 2018, Advance Article
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    Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA

    N. N. Patwardhan, Z. Cai, C. N. Newson and A. E. Hargrove, Org. Biomol. Chem., 2018, Advance Article , DOI: 10.1039/C8OB02467G

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