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Fluorogenic “photoclick” labelling of DNA using a Cy3 dye

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Abstract

Two 2′-deoxyuridines as new building blocks for automated DNA synthesis carry a small aryltetrazole as “photoclickable” group at their 5-positions. The postsynthetic “photoclick” labeling of such presynthesized DNA using a maleimide-modified Cy3 dye shows an up to 17-fold fluorogenicity due to an energy transfer between the pyrazoline moiety and the Cy3 fluorophore in the DNA products. This concept is also applicable to other maleimide-modified dyes.

Graphical abstract: Fluorogenic “photoclick” labelling of DNA using a Cy3 dye

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Publication details

The article was received on 23 Aug 2018, accepted on 02 Oct 2018 and first published on 03 Oct 2018


Article type: Communication
DOI: 10.1039/C8OB02068J
Citation: Org. Biomol. Chem., 2018, Advance Article
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    Fluorogenic “photoclick” labelling of DNA using a Cy3 dye

    B. Lehmann and H. Wagenknecht, Org. Biomol. Chem., 2018, Advance Article , DOI: 10.1039/C8OB02068J

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