Issue 41, 2018

Fluorogenic “photoclick” labelling of DNA using a Cy3 dye

Abstract

Two 2′-deoxyuridines as new building blocks for automated DNA synthesis carry a small aryltetrazole as “photoclickable” group at their 5-positions. The postsynthetic “photoclick” labeling of such presynthesized DNA using a maleimide-modified Cy3 dye shows an up to 17-fold fluorogenicity due to an energy transfer between the pyrazoline moiety and the Cy3 fluorophore in the DNA products. This concept is also applicable to other maleimide-modified dyes.

Graphical abstract: Fluorogenic “photoclick” labelling of DNA using a Cy3 dye

Supplementary files

Article information

Article type
Communication
Submitted
23 Aug 2018
Accepted
02 Oct 2018
First published
03 Oct 2018

Org. Biomol. Chem., 2018,16, 7579-7582

Fluorogenic “photoclick” labelling of DNA using a Cy3 dye

B. Lehmann and H. Wagenknecht, Org. Biomol. Chem., 2018, 16, 7579 DOI: 10.1039/C8OB02068J

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