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Issue 43, 2018
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Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

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Abstract

Optical biosensing based on the activation of fluorescent reporters offers a powerful methodology for the real-time molecular interrogation of pathology. Here we report a first-in-class, bimodal fluorescent reporter strategy for the simultaneous and highly specific detection of two independent proteases (thrombin and matrix metalloproteases (MMPs)) pivotal in the fibroproliferative process surrounding lung cancer, based on a dual, multiplexing, peptide FRET system. This sophisticated synthetic smartprobe, with a molecular weight of 6 kDa, contains two independent fluorophores and quenchers that generate photonic signatures at two specific wavelengths upon activation by target enzymes within human lung cancer tissue.

Graphical abstract: Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

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Publication details

The article was received on 25 Jul 2018, accepted on 22 Aug 2018 and first published on 23 Aug 2018


Article type: Paper
DOI: 10.1039/C8OB01790E
Citation: Org. Biomol. Chem., 2018,16, 8056-8063
  • Open access: Creative Commons BY license
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    Bimodal fluorogenic sensing of matrix proteolytic signatures in lung cancer

    A. Megia-Fernandez, B. Mills, C. Michels, S. V. Chankeshwara, N. Krstajić, C. Haslett, K. Dhaliwal and M. Bradley, Org. Biomol. Chem., 2018, 16, 8056
    DOI: 10.1039/C8OB01790E

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