Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 19, 2018
Previous Article Next Article

Chemo-enzymatic synthesis of isotopically labeled nicotinamide riboside

Author affiliations


As a cofactor for numerous reactions, NAD+ is found widely dispersed across many maps of cellular metabolism. This core redox role alone makes the biosynthesis of NAD+ of great interest. Recent studies have revealed new biological roles for NAD+ as a substrate for diverse enzymes that regulate a broad spectrum of key cellular tasks. These NAD+-consuming enzymes further highlight the importance of understanding NAD+ biosynthetic pathways. In this study, we developed a chemo-enzymatic synthesis of isotopically labeled NAD+ precursor, nicotinamide riboside (NR). The synthesis of NR isotopomers allowed us to unambiguously determine that NR is efficiently converted to NAD+ in the cellular environment independent of degradation to nicotinamide, and it is incorporated into NAD+ in its intact form. The versatile synthetic method along with the isotopically labeled NRs will provide powerful tools to further decipher the important yet complicated NAD+ metabolism.

Graphical abstract: Chemo-enzymatic synthesis of isotopically labeled nicotinamide riboside

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Mar 2018, accepted on 23 Apr 2018 and first published on 23 Apr 2018

Article type: Paper
DOI: 10.1039/C8OB00552D
Citation: Org. Biomol. Chem., 2018,16, 3662-3671

  •   Request permissions

    Chemo-enzymatic synthesis of isotopically labeled nicotinamide riboside

    A. Tran, R. Yokose and Y. Cen, Org. Biomol. Chem., 2018, 16, 3662
    DOI: 10.1039/C8OB00552D

Search articles by author