Jump to main content
Jump to site search

Issue 47, 2018
Previous Article Next Article

Adhesion strategies of Dictyostelium discoideum – a force spectroscopy study

Author affiliations

Abstract

Biological adhesion is essential for all motile cells and generally limits locomotion to suitably functionalized substrates displaying a compatible surface chemistry. However, organisms that face vastly varying environmental challenges require a different strategy. The model organism Dictyostelium discoideum (D.d.), a slime mould dwelling in the soil, faces the challenge of overcoming variable chemistry by employing the fundamental forces of colloid science. To understand the origin of D.d. adhesion, we realized and modified a variety of conditions for the amoeba comprising the absence and presence of the specific adhesion protein Substrate Adhesion A (sadA), glycolytic degradation, ionic strength, surface hydrophobicity and strength of van der Waals interactions by generating tailored model substrates. By employing AFM-based single cell force spectroscopy we could show that experimental force curves upon retraction exhibit two regimes. The first part up to the critical adhesion force can be described in terms of a continuum model, while the second regime of the curve beyond the critical adhesion force is governed by stochastic unbinding of individual binding partners and bond clusters. We found that D.d. relies on adhesive interactions based on EDL-DLVO (Electrical Double Layer-Derjaguin–Landau–Verwey–Overbeek) forces and contributions from the glycocalix and specialized adhesion molecules like sadA. This versatile mechanism allows the cells to adhere to a large variety of natural surfaces under various conditions.

Graphical abstract: Adhesion strategies of Dictyostelium discoideum – a force spectroscopy study

Back to tab navigation

Supplementary files

Publication details

The article was received on 31 Aug 2018, accepted on 01 Nov 2018 and first published on 02 Nov 2018


Article type: Paper
DOI: 10.1039/C8NR07107A
Citation: Nanoscale, 2018,10, 22504-22519
  • Open access: Creative Commons BY license
  •   Request permissions

    Adhesion strategies of Dictyostelium discoideum – a force spectroscopy study

    N. Kamprad, H. Witt, M. Schröder, C. T. Kreis, O. Bäumchen, A. Janshoff and M. Tarantola, Nanoscale, 2018, 10, 22504
    DOI: 10.1039/C8NR07107A

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements