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Glucose oxidase-loaded liposomes for in-situ amplified signal of electrochemical immunoassay on a handheld pH meter

Abstract

Methods based on pH meter have developed for immunoassays, but most involve the low sensitivity and weak detectable signal and thus are unsuitable for routine use. Herein, a simple and portable electrochemical immunoassay was designed for sensitive screening of disease-related biomarker, neuron-specific enolase (NSE), by using glucose oxidase-loaded liposome (GOx-LS) for the signal amplification. Initially, a sandwiched immunoreaction was carried out on rabbit anti-human monoclonal NSE antibody-coated microplate with polyclonal rabbit anti-human NSE antibody- labeled GOx-LS. Thereafter, the carried GOx-LS accompanying the immunocomplex could oxidize glucose into gluconic acid and hydrogen peroxide, thereby resulting in the pH change of detection solution, which could be registered on a handheld pH meter. Relative to GOx-labeled strategy, use of GOx-LS could amplify the signal of electrochemical immunosensing platform. Under optimum conditions, GOx-LS-based immunoassay exhibited good pH responses toward target NSE within the dynamic linear range of 0.01 – 100 ng mL-1 at a detection limit of 8.9 pg mL-1. The coefficient of variations for the reproducibility and precision of this method were less than 7.9% and 12.7% for the single-batch and different-batch identification, respectively. High specificity and acceptable stability were acquired to determine target NSE. For the detection of human serum specimens, well-matched results were provided between pH-based immunoassay and commercial Electrochemiluminescent Automatic Analyzer.

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Publication details

The article was received on 06 Nov 2018, accepted on 07 Dec 2018 and first published on 08 Dec 2018


Article type: Paper
DOI: 10.1039/C8NJ05637D
Citation: New J. Chem., 2018, Accepted Manuscript
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    Glucose oxidase-loaded liposomes for in-situ amplified signal of electrochemical immunoassay on a handheld pH meter

    J. Liang, J. Wang, L. Zhang, S. Wang, C. Yao and Z. Zhang, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ05637D

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