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Lysosome-targeted two-photon fluorescent probes for rapid detection of H2S in live cells

Abstract

Two novel two-photon fluorescent probes (BHNP-DA and M2) were designed and synthesized. They show good selectivity and high sensitivity to H2S. The response of the probes to H2S in Tris-HCl buffer was very fast (50 s). The reaction mechanism of probe BHNP-DA is based on a hydrogen sulfide mediated tandem disulfide exchange, followed by nucleophilic substitution-cyclization reaction and the fluorescence is turned on. The dual-nucleophilicity reaction of H2S could not be observed on the probe M2 whose fluorophore group is linked by amide and fluorescence is turned off. Furthermore, probes were successfully applied in living MCF-7 cells fluorescence imaging. The colocalization experiments illuminate the probe BHNP-DA can target lysosome well.

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Publication details

The article was received on 25 Oct 2018, accepted on 07 Dec 2018 and first published on 08 Dec 2018


Article type: Paper
DOI: 10.1039/C8NJ05419C
Citation: New J. Chem., 2018, Accepted Manuscript
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    Lysosome-targeted two-photon fluorescent probes for rapid detection of H2S in live cells

    G. Li, S. Ma, J. tang and Y. Ye, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ05419C

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