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Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor: Complementary Match of Supramolecular and Dynamic Chemistry

Abstract

Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for Trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the strong interaction of bisphosphonate with lysine/arginine residues on the surface of Trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.

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Publication details

The article was received on 15 Oct 2018, accepted on 03 Dec 2018 and first published on 04 Dec 2018


Article type: Paper
DOI: 10.1039/C8NJ05238G
Citation: New J. Chem., 2018, Accepted Manuscript
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    Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor: Complementary Match of Supramolecular and Dynamic Chemistry

    G. Ertürk, M. akhoundian, K. Kujawski, S. Shinde, S. Y. Yeung, M. Hedström, T. Schrader, B. Mattiasson and B. Sellergren, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ05238G

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