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Poly(β-cyclodextrin) enhanced fluorescence coupled with specific reaction for amplified detection of GSH and trypsin activity

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Abstract

Up to now, the successful fabrication of efficient specific reaction-based sensing systems for amplification signal-enhanced detection of reactive species remains an unsolved problem. To address this issue, we describe herein a universal β-cyclodextrin polymer (β-CDP)-based sensing platform that uses an amplified fluorescence assay for sensitive detection of glutathione (GSH) and trypsin activity. More specifically, the negatively charged peptide Py-GC adsorbed on the surfaces of MnO2 nanotubes via electrostatic interaction. Upon addition of GSH, MnO2 was reduced to Mn2+ and Py-GC was separated far from the nanoquencher, generating an activated fluorescence signal. It was worth noting that free pyrene could easily enter the hydrophobic cavity of β-CDP, accompanied by significant fluorescence enhancement. The nanoprobe exhibits high sensitivity and selectivity to GSH as well as excellent biocompatibility, making it a promising probe for imaging of endogenous GSH in living cells. As a proof-of-principle, we use this approach to quantify trypsin activity on the basis of the trypsin-catalyzed hydrolysis reaction of cytochrome c (Cyt c) in human serum. As a universal βCDP-based signal amplification reporter, this prototype can be applied to a sensitive and versatile sensing platform for multi-analyses by selecting suitable switched-reagents, thus inspiring the new strategies for bioanalysis.

Graphical abstract: Poly(β-cyclodextrin) enhanced fluorescence coupled with specific reaction for amplified detection of GSH and trypsin activity

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Publication details

The article was received on 24 Aug 2018, accepted on 02 Oct 2018 and first published on 03 Oct 2018


Article type: Paper
DOI: 10.1039/C8NJ04325F
Citation: New J. Chem., 2018, Advance Article
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    Poly(β-cyclodextrin) enhanced fluorescence coupled with specific reaction for amplified detection of GSH and trypsin activity

    H. Yan, Q. Gao, Y. Liu, W. Ren, J. Shangguan, X. Yang and K. Li, New J. Chem., 2018, Advance Article , DOI: 10.1039/C8NJ04325F

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