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Synthesis, characterisation, crystal structure and biological studies of palladium(II) complexes containing 5-(2-hydroxy-3-methoxy-phenyl)-2,4-dihydro[1,2,4]triazole-3-thione derivatives

Abstract

A series of palladium(II) complexes containing 5-(2-hydroxy-3-methoxy-phenyl)-2,4 dihydro[1,2,4] triazole-3-thione derivatives were obtained from the 2:2:1 stoichiometric reactions of K2[PdCl4], 3-methoxysalicylaldehyde-4(N)-substituted thiosemicarbazone [H2L1-H2L4] and 1,3-bis(diphenylphosphino)propane [dppp]. The complexes were characterized by various spectroscopic techniques (IR, electronic, 1H-NMR and ESI-MS). The crystal structure of the complexes 2 and 4 has been determined by X-ray diffraction. The binding affinity and binding mode of the palladium complexes (1-4) towards calf-thymus (CT) DNA and BSA (Bovine Serum Albumin) were studied by using UV-Vis and fluorescence emission spectroscopy. These results showed that the complexes binding to DNA through electrostatic and quenching of the complexes is static. Further, they cleaved supercoiled DNA pBR322 without any additional external agents. The alterations in the secondary structure of BSA by the Pd(II) complexes were confirmed by synchronous and three dimensional fluorescence spectroscopy. Cytotoxicity of the complexes were evaluated by MTT assay in MCF-7 cell line and complex 3 exhibited significant activity (IC50= 5.60±0.04 µM) which was comparable with the other complexes. The morphological changes assessed by acridine orange and ethidium bromide staining revealed that the cell death occurred by apoptosis.

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Publication details

The article was received on 25 Jul 2018, accepted on 09 Oct 2018 and first published on 10 Oct 2018


Article type: Paper
DOI: 10.1039/C8NJ03714K
Citation: New J. Chem., 2018, Accepted Manuscript
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    Synthesis, characterisation, crystal structure and biological studies of palladium(II) complexes containing 5-(2-hydroxy-3-methoxy-phenyl)-2,4-dihydro[1,2,4]triazole-3-thione derivatives

    S. Arumugam, F. Dallemer and R. Prabhakaran, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ03714K

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