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Insights into Apatite Mineralization Potential of Thermally Processed Nanocrystalline Ca10-xFex(PO4)6(OH)2

Abstract

Understanding the crystalline property of nano-hydroxyapatite (nHAp) from amorphous material is a quite complex process involving thermal treatment, size and charge of ions and arrangement of ions or molecules into an ordered lattice, consequently influences structural, morphological and their biological properties. Varying ratios of iron (Fe) incorporated nano-hydroxyapatite (Fe-nHAp) subjected to different thermal treatments mediated apatite mineralization potential on non-cellular and cellular conditions are explored herein. An increase in thermal treatment induces a phase transition from nHAp to β-TCP, which gradually increased due to Fe incorporation and declined the size aspect ratio followed by irregular morphology evidenced by XRD and high resolution TEM analyses. Mineralization potential in non-cellular condition is considerably influenced by the rate of calcium (Ca), phosphate (PO4) and Fe ionic releases. Even if apatite layer formed by nanocrystalline Fe-nHAp can endow bioactivity to host bone tissue, however, it is important to address other biological activities. In line with this, in cellular conditions, mineralization potential of Ca, P and Fe ions were assessed on Saos-2 cells by alkaline phosphatase (ALP) activity, gene expression and calcium nodule formation that shows enhanced potential for mineralization upto certain ratio of Fe incorporation as well as thermal treatment. Thus could be perceived that newly formed bone-like apatite layer formation was insufficient for the success of biomaterials in tissue regeneration, rather optimal levels of Ca, P and Fe ions are essential to up-regulate osteoblasts, which may promote new tissue regeneration.

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Publication details

The article was received on 18 Jul 2018, accepted on 03 Dec 2018 and first published on 06 Dec 2018


Article type: Paper
DOI: 10.1039/C8NJ03579B
Citation: New J. Chem., 2018, Accepted Manuscript
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    Insights into Apatite Mineralization Potential of Thermally Processed Nanocrystalline Ca10-xFex(PO4)6(OH)2

    G. Radha, B. Venkatesan, P. Rajashree, E. Vellaichamy and B. Subramanian, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ03579B

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