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Issue 10, 2018
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Biosynthesis and isolation of selenoneine from genetically modified fission yeast

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Abstract

Selenoneine, a naturally occurring form of selenium, is the selenium analogue of ergothioneine, a sulfur species with health relevance not only as a purported antioxidant but likely also beyond. Selenoneine has been speculated to exhibit similar effects. To study selenoneine's health properties as well as its metabolic transformation, the pure compound is required. Chemical synthesis of selenoneine, however, is challenging and biosynthetic approaches have been sought. We herein report the biosynthesis and isolation of selenoneine from genetically modified fission yeast Schizosaccharomyces pombe grown in a medium containing sodium selenate. After cell lysis and extraction with methanol, selenoneine was purified by three consecutive preparative reversed-phase HPLC steps. The product obtained at the mg level was characterised by high resolution mass spectrometry, NMR and HPLC/ICPMS. Biosynthesis was found to be a promising alternative to chemical synthesis, and should be suitable for upscaling to produce higher amounts of this important selenium species in the future.

Graphical abstract: Biosynthesis and isolation of selenoneine from genetically modified fission yeast

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Publication details

The article was received on 24 Jul 2018, accepted on 17 Sep 2018 and first published on 17 Sep 2018


Article type: Paper
DOI: 10.1039/C8MT00200B
Citation: Metallomics, 2018,10, 1532-1538
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    Biosynthesis and isolation of selenoneine from genetically modified fission yeast

    N. G. Turrini, N. Kroepfl, K. B. Jensen, T. C. Reiter, K. A. Francesconi, T. Schwerdtle, W. Kroutil and D. Kuehnelt, Metallomics, 2018, 10, 1532
    DOI: 10.1039/C8MT00200B

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