Crystallographic evidence for unexpected selective tyrosine hydroxylations in an aerated achiral Ru–papain conjugate

Mickaël V. Cherrier; Patricia Amara; Barisa Talbi; Michèle Salmain; Juan C. Fontecilla-Camps

doi:10.1039/C8MT00160J

Crystallographic evidence for unexpected selective tyrosine hydroxylations in an aerated achiral Ru–papain conjugate†

Mickaël V. Cherrier,

^a Patricia Amara,

^a Barisa Talbi,^b Michèle Salmain

^b and Juan C. Fontecilla-Camps

*^a

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* Corresponding authors

^a Univ. Grenoble Alpes, CEA, CNRS, IBS, Metalloproteins, F-38000 Grenoble, France
E-mail: juan.fontecilla@ibs.fr

^b Sorbonne Université, CNRS, Institut Parisien de Chimie Moléculaire (IPCM), 4 place Jussieu, Paris, France

Abstract

The X-ray structure of an aerated achiral Ru–papain conjugate has revealed the hydroxylation of two tyrosine residues found near the ruthenium ion. The most likely mechanism involves a ruthenium-bound superoxide as the reactive species responsible for the first hydroxylation and the resulting high valent Ru(IV) [double bond, length as m-dash] O species for the second one.

Metallomics

Crystallographic evidence for unexpected selective tyrosine hydroxylations in an aerated achiral Ru–papain conjugate†

Abstract

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