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Lipidomics reveals insights on the biological effects of copper oxide nanoparticles in a human colon carcinoma cell line

Abstract

Engineered nanomaterials have unique properties compared to their bulk counterparts. Copper oxide nanoparticles (CuO NPs) are one example of nanomaterials used in a wide range of consumer products due to their conductivity and biocidal properties. While CuO NPs can induce toxicity in various organisms, their interactions with different organisms and how they affect cellular homeostasis is yet to be fully understood. In this work, the toxicity of CuO NPs was evaluated in different human cell lines (colorectal carcinoma, cervical cancer, embryonic kidney, and lung fibroblast), showing a dose-dependent toxicity. An untargeted lipidomics approach using liquid chromatography-quadrupole time of flight mass spectrometry was employed in a human colon carcinoma cell line to investigate the impact of CuO NP exposure at the cellular level. A 24-hour CuO NP exposure at 2.5 and 5 µg/mL resulted in an upregulation of different metabolites. Specifically, triacylglycerols, phosphatidylcholines, and ceramides accumulated. The most profound increase in a dose dependent manner was ceramides, specifically C18:0 , C18:1, and C22:0 species, with accumulations up to ~10 fold change. Further experiments suggested that activation of autophagy and oxidative stress could be responsible for the toxicity observed in these cell lines. Increases in the level of glutathione oxide (~ 7 fold change) also supported the activation of oxidative stress upon CuO NP treatment. Based on the changes in different metabolites induced by CuO NP exposure and previous studies from our laboratory, we propose that autophagy oxidative stress might play a role in CuO NP-induced toxicity.

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Publication details

The article was received on 17 Jul 2018, accepted on 04 Dec 2018 and first published on 05 Dec 2018


Article type: Research Article
DOI: 10.1039/C8MO00162F
Citation: Mol. Omics, 2018, Accepted Manuscript
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    Lipidomics reveals insights on the biological effects of copper oxide nanoparticles in a human colon carcinoma cell line

    N. G. Chavez Soria, D. S. Aga and G. E. Atilla-Gokcumen, Mol. Omics, 2018, Accepted Manuscript , DOI: 10.1039/C8MO00162F

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