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Digital polymerase chain reaction technology – recent advances and future perspectives

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Abstract

Digital polymerase chain reaction (dPCR) technology has remained a “hot topic” in the last two decades due to its potential applications in cell biology, genetic engineering, and medical diagnostics. Various advanced techniques have been reported on sample dispersion, thermal cycling and output monitoring of digital PCR. However, a fully automated, low-cost and handheld digital PCR platform has not been reported in the literature. This paper attempts to critically evaluate the recent developments in techniques for sample dispersion, thermal cycling and output evaluation for dPCR. The techniques are discussed in terms of hardware simplicity, portability, cost-effectiveness and suitability for automation. The present paper also discusses the research gaps observed in each step of dPCR and concludes with possible improvements toward portable, low-cost and automatic digital PCR systems.

Graphical abstract: Digital polymerase chain reaction technology – recent advances and future perspectives

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Publication details

The article was received on 19 Sep 2018, accepted on 28 Oct 2018 and first published on 29 Oct 2018


Article type: Critical Review
DOI: 10.1039/C8LC00990B
Citation: Lab Chip, 2018, Advance Article
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    Digital polymerase chain reaction technology – recent advances and future perspectives

    K. R. Sreejith, C. H. Ooi, J. Jin, D. V. Dao and N. Nguyen, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00990B

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