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Issue 22, 2018
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SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA)

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Abstract

Quantitative detection of RNA is important in molecular biology and clinical diagnostics. Nucleic acid sequence-based amplification (NASBA), a single-step method to amplify single-stranded RNA, is attractive for use in point-of-care (POC) diagnostics because it is an isothermal technique that is as sensitive as RT-PCR with a shorter reaction time. However, NASBA is limited in its ability to provide accurate quantitative information, such as viral load or RNA copy number. Here we test a digital format of NASBA (dNASBA) using a self-digitization (SD) chip platform, and apply it to quantifying HIV-1 RNA. We demonstrate that dNASBA is more sensitive and accurate than the real-time quantitative NASBA, and can be used to quantify HIV-1 RNA in plasma samples. Digital NASBA is thus a promising POC diagnostics tool for use in resource-limited settings.

Graphical abstract: SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA)

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Publication details

The article was received on 10 Sep 2018, accepted on 12 Oct 2018 and first published on 17 Oct 2018


Article type: Paper
DOI: 10.1039/C8LC00956B
Citation: Lab Chip, 2018,18, 3501-3506
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    SD-chip enabled quantitative detection of HIV RNA using digital nucleic acid sequence-based amplification (dNASBA)

    J. Wang, J. E. Kreutz, A. M. Thompson, Y. Qin, A. M. Sheen, J. Wang, L. Wu, S. Xu, M. Chang, D. N. Raugi, R. A. Smith, G. S. Gottlieb and D. T. Chiu, Lab Chip, 2018, 18, 3501
    DOI: 10.1039/C8LC00956B

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